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PCR

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PCR
Name: Gal-Oalb-2 Type: Real-Time PCR System: QuaNtitative
Genetic Element: Ovalbumin Ampl.Length: 103 GenomSize: 1.25 AnnTemp: 64
Taxonomy: Chicken PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 4 Organisation: BfR
Validation: NoValidationAvailable Sequence: J00895
Remarks: Reference Sequence: J00895 M24999
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Gal-Oalb-2f Sense TTCTg TATCA AgCAC ATCgC AA 22 65.4 56.7 62 40.9 8197-8218
Gal-Oalb-2r Antisense gTCTT gTTgg AAggg ACAgA gTT 23 66.5 57.7 68 47.8 8299-8277
Gal-Oalb-2probe Marker/Probe CAACg CCgTT CTCTT CTTTg gCAgA 25 72.1 67.4 76 52.0 8220-8244
Gal-Oalb-2f Amplicon ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc 103 64 88.8 296 43.7 8197-8299
Sequence
NCBI-Acc: J00895 M24999 Sequence Description: Gallus gallus ovalbumin gene, complete cds.
BaseCount: 2994 a 1749 c 1721 g 2742 t
DNA-Sequence: J00895.txt AminoAcid-Sequence: J00895.txt
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