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PCR |
Name: |
Gal-Oalb-2 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Ovalbumin |
Ampl.Length: |
103 |
GenomSize: |
1.25 |
AnnTemp: |
64 |
Taxonomy: |
Chicken |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
4 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
J00895 |
Remarks: |
Reference Sequence: J00895 M24999 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Gal-Oalb-2f |
Sense |
TTCTg TATCA AgCAC ATCgC AA |
22 |
65.4 |
56.7 |
62 |
40.9 |
8197-8218 |
Gal-Oalb-2r |
Antisense |
gTCTT gTTgg AAggg ACAgA gTT |
23 |
66.5 |
57.7 |
68 |
47.8 |
8299-8277 |
Gal-Oalb-2probe |
Marker/Probe |
CAACg CCgTT CTCTT CTTTg gCAgA |
25 |
72.1 |
67.4 |
76 |
52.0 |
8220-8244 |
Gal-Oalb-2f |
Amplicon |
ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc |
103 |
64 |
88.8 |
296 |
43.7 |
8197-8299 |
Sequence |
NCBI-Acc: |
J00895 M24999 |
Sequence Description: |
Gallus gallus ovalbumin gene, complete cds. |
BaseCount: |
2994 a 1749 c 1721 g 2742 t |
DNA-Sequence: |
J00895.txt |
AminoAcid-Sequence: |
J00895.txt |
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