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You are here: Core Forms > Search PCR > PCR
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PCR |
Name: |
WBR |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
Intron of trnL gene |
Ampl.Length: |
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GenomSize: |
|
AnnTemp: |
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Taxonomy: |
Wheat |
PCR Description: |
Taxon: Detection of Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233. |
Temp. Time Program: |
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Device: |
|
Master Mix: |
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nPrimers: |
2 |
Organisation: |
VUP |
Validation: |
NoValidationAvailable |
Sequence: |
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Remarks: |
Taxon: Only Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233. |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
WBR11 |
Sense |
ggTAA CTTCC AAATT CAgAg AAAC |
24 |
62.7 |
53.9 |
66 |
37.5 |
|
WBR13 |
Antisense |
TCTCT AATTT AgAAT TAgAA ggAA |
24 |
55.7 |
46.9 |
60 |
25.0 |
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