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PCR
Name: WBR Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: Intron of trnL gene Ampl.Length: GenomSize: AnnTemp:
Taxonomy: Wheat PCR Description: Taxon: Detection of Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233. Temp. Time Program:
Device: Master Mix: nPrimers: 2 Organisation: VUP
Validation: NoValidationAvailable Sequence:
Remarks: Taxon: Only Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233.
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
WBR11 Sense ggTAA CTTCC AAATT CAgAg AAAC 24 62.7 53.9 66 37.5
WBR13 Antisense TCTCT AATTT AgAAT TAgAA ggAA 24 55.7 46.9 60 25.0
Publications
Short Title Type Date Download
Journal: WBR_gluten-free_QuaNt ArticleInJournal 2001
SOP: WBR_gluten-containing_powder CollaborativeStudy July 2004 CollStudyWBR_p9.pdf
SOP: WBR_gluten-containing SOP March 2004 SOP_GlutenWBR_detectionPCR_p9.pdf
Val: WBR_QuaL Validation 2004? Validation_wheat-rye-barley_p5.pdf
PCR detection of Wheat - Rye – Barley - December 2004 RT1-protocol_TaqBead.pdf
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