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PCR |
Name: |
Hsp |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
GM-CSF (?) |
Ampl.Length: |
180 |
GenomSize: |
3.15 |
AnnTemp: |
58 |
Taxonomy: |
Horse |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
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nPrimers: |
3 |
Organisation: |
Eurofins |
Validation: |
NoValidationAvailable |
Sequence: |
AY040203 |
Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Hspf-2 |
Sense |
CAACA gTAgT gACAC TgCTg CTATC |
25 |
65.4 |
56.7 |
74 |
48.0 |
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Hspr-3 |
Antisense |
CTCCT CggCg TCAAA Cg |
17 |
66.8 |
58.1 |
56 |
64.7 |
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HspMGB1 |
Marker/Probe |
AgggA gggAg CACgg |
15 |
64.0 |
55.2 |
52 |
73.3 |
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Sequence |
NCBI-Acc: |
AY040203 |
Sequence Description: |
Equus caballus granulocyte-macrophage colony-stimulating-factor (GM-CSF) mRNA, partial cds. |
BaseCount: |
220 a 193 c 176 g 190 t |
DNA-Sequence: |
AY040203.txt |
AminoAcid-Sequence: |
AY040203.txt |
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