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PCR
Name: Hsp Type: Real-Time PCR System: QuaNtitative
Genetic Element: GM-CSF (?) Ampl.Length: 180 GenomSize: 3.15 AnnTemp: 58
Taxonomy: Horse PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 3 Organisation: Eurofins
Validation: NoValidationAvailable Sequence: AY040203
Remarks:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Hspf-2 Sense CAACA gTAgT gACAC TgCTg CTATC 25 65.4 56.7 74 48.0
Hspr-3 Antisense CTCCT CggCg TCAAA Cg 17 66.8 58.1 56 64.7
HspMGB1 Marker/Probe AgggA gggAg CACgg 15 64.0 55.2 52 73.3
Sequence
NCBI-Acc: AY040203 Sequence Description: Equus caballus granulocyte-macrophage colony-stimulating-factor (GM-CSF) mRNA, partial cds.
BaseCount: 220 a 193 c 176 g 190 t
DNA-Sequence: AY040203.txt AminoAcid-Sequence: AY040203.txt
Publications
Short Title Type Date Download
Val: Horse_QuaL_PCR Validation 2004 ValidationPCRhorse_p6.pdf
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