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PCR |
Name: |
Ostr |
Type: |
Real-Time PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
Mitochondrial Cytochrome b |
Ampl.Length: |
92 |
GenomSize: |
2.16 |
AnnTemp: |
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Taxonomy: |
Ostrich |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
2 |
Organisation: |
Eurofins |
Validation: |
NoValidationAvailable |
Sequence: |
AF069431 |
Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Ostrf-1 |
Sense |
CTACT ATCCC TAgCA TTCTT CTCg |
24 |
62.8 |
54.1 |
70 |
45.8 |
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Ostrr-2 |
Antisense |
TgATA Tgggg AggCg Tgg |
18 |
66.8 |
58.0 |
58 |
61.1 |
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Sequence |
NCBI-Acc: |
AF069431 |
Sequence Description: |
Struthio camelus cytochrome b gene, partial cds; and tRNA-Thr gene, partial sequence, mitochondrial genes for mitochondrial products. |
BaseCount: |
289 a 354 c 139 g 286 t |
DNA-Sequence: |
AF069431.txt |
AminoAcid-Sequence: |
AF069431.txt |
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