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PCR

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PCR
Name: Ostr Type: Real-Time PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 92 GenomSize: 2.16 AnnTemp:
Taxonomy: Ostrich PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 2 Organisation: Eurofins
Validation: NoValidationAvailable Sequence: AF069431
Remarks:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Ostrf-1 Sense CTACT ATCCC TAgCA TTCTT CTCg 24 62.8 54.1 70 45.8
Ostrr-2 Antisense TgATA Tgggg AggCg Tgg 18 66.8 58.0 58 61.1
Sequence
NCBI-Acc: AF069431 Sequence Description: Struthio camelus cytochrome b gene, partial cds; and tRNA-Thr gene, partial sequence, mitochondrial genes for mitochondrial products.
BaseCount: 289 a 354 c 139 g 286 t
DNA-Sequence: AF069431.txt AminoAcid-Sequence: AF069431.txt
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