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PCR |
Name: |
Macrop |
Type: |
Real-Time PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
Mitochondrial Cytochrome b |
Ampl.Length: |
164 |
GenomSize: |
4.21/3.13 |
AnnTemp: |
|
Taxonomy: |
Kangaroo |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
2 |
Organisation: |
Eurofins |
Validation: |
NoValidationAvailable |
Sequence: |
U87137 |
Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Macropf-1 |
Sense |
CTTAC CgTTT ATTAT TACAg CCTTA gTAC |
29 |
64.1 |
55.4 |
78 |
34.5 |
|
Macropr-1 |
Antisense |
ggACA AggAg TATAA gTATA ARgCC A |
26 |
62.4 |
54.3 |
70 |
38.5 |
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Sequence |
NCBI-Acc: |
U87137 |
Sequence Description: |
Macropus giganteus cytochrome b (cytb) gene, mitochondrial gene encoding mitochondrial protein, complete cds. |
BaseCount: |
343 a 363 c 142 g 298 t |
DNA-Sequence: |
U87137.txt |
AminoAcid-Sequence: |
U87137.txt |
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