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PCR |
Name: |
CytB-uni |
Type: |
PCR-RFLP |
System: |
Sequencing |
Genetic Element: |
Mitochondrial Cytochrome b |
Ampl.Length: |
1143 |
GenomSize: |
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AnnTemp: |
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Taxonomy: |
Animals |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
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nPrimers: |
2 |
Organisation: |
UU-FDG |
Validation: |
NoValidationAvailable |
Sequence: |
Cytochrome b |
Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
CytB-uni-F |
Sense |
CATTT ATTgA CCTCC CAgCC CC |
22 |
70.5 |
61.7 |
68 |
54.5 |
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CytB-uni-R |
Antisense |
AgCCT CCTCA gATTC ATTCg AC |
22 |
66.1 |
57.4 |
66 |
50.0 |
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Sequence |
NCBI-Acc: |
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Sequence Description: |
Sequence-Alignment of 218 Mitochondrial Cytochrome b-Sequences of 218 animal species. |
BaseCount: |
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DNA-Sequence: |
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AminoAcid-Sequence: |
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