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PCR |
Name: |
Bos-pde0 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
104 |
GenomSize: |
|
AnnTemp: |
60 |
Taxonomy: |
Cattle |
PCR Description: |
|
Temp. Time Program: |
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Device: |
|
Master Mix: |
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nPrimers: |
4 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
M27541 |
Remarks: |
Corr.Primers: bos-f, bos-r and bos-probe. Reference Sequence: M27541 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-f |
Sense |
ACTCC TACCC ATCAT gCAgA T |
21 |
63.1 |
54.3 |
62 |
47.6 |
3007-3027 |
Bos-r |
Antisense |
TgTTT TTAAA TATTT CAgCT AAgAA AAA |
28 |
63.1 |
54.3 |
66 |
17.9 |
3110-3083 |
Bos-probe |
Marker/Probe |
AACAT CAggA TTTTT gCTgC ATTTg C |
26 |
72.1 |
63.8 |
72 |
38.5 |
3031-3056 |
Bos-Amplicon |
Amplicon |
ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA |
104 |
60 |
82.8 |
276 |
32.7 |
3007-3110 |
Sequence |
NCBI-Acc: |
M27541 |
Sequence Description: |
Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds. |
BaseCount: |
887 a 783 c 798 g 733 t |
DNA-Sequence: |
M27541.txt |
AminoAcid-Sequence: |
M27541.txt |
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