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PCR |
Name: |
Bos-pde |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
154 |
GenomSize: |
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AnnTemp: |
60 |
Taxonomy: |
Cattle |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
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nPrimers: |
4 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
M27541 |
Remarks: |
Reference Sequence: M27541 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-pde-f |
Sense |
ACACA ATgAA TTgAC TCCTA CCC |
23 |
64.1 |
55.4 |
66 |
43.5 |
2994-3016 |
Bos-pde-r |
Antisense |
ggTgC gTAAT ggAgg AgTC |
19 |
63.1 |
54.3 |
60 |
57.9 |
3147-3129 |
Bos-pde-probe |
Marker/Probe |
ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC |
33 |
71.9 |
63.1 |
86 |
30.3 |
3124-3092 |
Bos-pde-Amplicon |
Amplicon |
acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC |
154 |
60 |
87.5 |
420 |
36.4 |
2994-3147 |
Sequence |
NCBI-Acc: |
M27541 |
Sequence Description: |
Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds. |
BaseCount: |
887 a 783 c 798 g 733 t |
DNA-Sequence: |
M27541.txt |
AminoAcid-Sequence: |
M27541.txt |
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