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PCR |
Name: |
Gal-Oalb |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Ovalbumin |
Ampl.Length: |
87 |
GenomSize: |
1.25 |
AnnTemp: |
64 |
Taxonomy: |
Chicken |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
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nPrimers: |
4 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
J00895 |
Remarks: |
Reference Sequence: J00895 M24999 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Gal-Oalb-f |
Sense |
ATCgC AACCA ACgCC gT |
17 |
69.4 |
60.6 |
54 |
58.8 |
8212-8228 |
Gal-Oalb-r |
Antisense |
TCTTg TTggA AgggA CAgAg TTT |
23 |
41.8 |
57.9 |
66 |
43.5 |
8298-8276 |
Gal-Oalb-probe |
Marker/Probe |
TTTgg CAgAT gTgTT TCCCC TTAAA AAgAA |
30 |
36.9 |
66.4 |
82 |
36.7 |
8236-8265 |
Gal-Oalb-f |
Amplicon |
ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GA |
87 |
64 |
87.8 |
250 |
43.7 |
8212-8298 |
Sequence |
NCBI-Acc: |
J00895 M24999 |
Sequence Description: |
Gallus gallus ovalbumin gene, complete cds. |
BaseCount: |
2994 a 1749 c 1721 g 2742 t |
DNA-Sequence: |
J00895.txt |
AminoAcid-Sequence: |
J00895.txt |
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