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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Chicken |
Gallus |
gallus |
Aves |
Galliformes |
Phasianidae |
Sequence |
NCBI-Acc: |
J00895 M24999 |
Sequence Description: |
Gallus gallus ovalbumin gene, complete cds. |
BaseCount: |
2994 a 1749 c 1721 g 2742 t |
DNA-Sequence: |
J00895.txt |
AminoAcid-Sequence: |
J00895.txt |
PCR |
Name: |
Gal-Oalb-2 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
J00895 |
Genetic Element: |
Ovalbumin |
Ampl.Length: |
103 |
GenomSize: |
1.25 |
AnnTemp: |
64 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Gal-Oalb-2f |
Sense |
TTCTg TATCA AgCAC ATCgC AA |
22 |
65.4 |
56.7 |
62 |
40.9 |
8197-8218 |
Gal-Oalb-2r |
Antisense |
gTCTT gTTgg AAggg ACAgA gTT |
23 |
66.5 |
57.7 |
68 |
47.8 |
8299-8277 |
Gal-Oalb-2probe |
Marker/Probe |
CAACg CCgTT CTCTT CTTTg gCAgA |
25 |
72.1 |
67.4 |
76 |
52.0 |
8220-8244 |
Gal-Oalb-2f |
Amplicon |
ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc |
103 |
64 |
88.8 |
296 |
43.7 |
8197-8299 |
Organisation Data |
Acronym: |
BfR
|
|
Phone: |
+49-30-8412-0 |
Partner Number: |
1 |
Fax: |
+41 308412 3685 |
Type: |
Authority |
e-Mail: |
j.zagon@bfr.bund.de |
Name: |
BfR-Federal Institute for Risk Assessment, Berlin |
Website: |
http://www.bfr.bund.de/ |
Umbrella Organisation: |
Bund, Germany (Bundesinstitut für Risikobewertung) |
Postal Address: |
Thielallee 88-92, D-14195 Berlin |
Country: |
Germany |
Remarks: |
Coordinator MolSpec-ID-project, Task Leader Workpackages 1.4 and 4 |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
DNA+protein |
ABI 7700+LC |
ABI 310 |
PE 2400 |
yes |
ELISA plate reader |
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