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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Cattle |
Bos |
taurus |
Eutheria |
Ruminantia |
Bovidae |
Sequence |
NCBI-Acc: |
M27541 |
Sequence Description: |
Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds. |
BaseCount: |
887 a 783 c 798 g 733 t |
DNA-Sequence: |
M27541.txt |
AminoAcid-Sequence: |
M27541.txt |
PCR |
Name: |
Bos-pde0 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
M27541 |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
104 |
GenomSize: |
|
AnnTemp: |
60 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-f |
Sense |
ACTCC TACCC ATCAT gCAgA T |
21 |
63.1 |
54.3 |
62 |
47.6 |
3007-3027 |
Bos-r |
Antisense |
TgTTT TTAAA TATTT CAgCT AAgAA AAA |
28 |
63.1 |
54.3 |
66 |
17.9 |
3110-3083 |
Bos-probe |
Marker/Probe |
AACAT CAggA TTTTT gCTgC ATTTg C |
26 |
72.1 |
63.8 |
72 |
38.5 |
3031-3056 |
Bos-Amplicon |
Amplicon |
ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA |
104 |
60 |
82.8 |
276 |
32.7 |
3007-3110 |
Organisation Data |
Acronym: |
BfR
|
|
Phone: |
+49-30-8412-0 |
Partner Number: |
1 |
Fax: |
+41 308412 3685 |
Type: |
Authority |
e-Mail: |
j.zagon@bfr.bund.de |
Name: |
BfR-Federal Institute for Risk Assessment, Berlin |
Website: |
http://www.bfr.bund.de/ |
Umbrella Organisation: |
Bund, Germany (Bundesinstitut für Risikobewertung) |
Postal Address: |
Thielallee 88-92, D-14195 Berlin |
Country: |
Germany |
Remarks: |
Coordinator MolSpec-ID-project, Task Leader Workpackages 1.4 and 4 |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
DNA+protein |
ABI 7700+LC |
ABI 310 |
PE 2400 |
yes |
ELISA plate reader |
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