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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Animals |
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Sequence |
NCBI-Acc: |
X56295 |
Sequence Description: |
S.scrofa mitochondrion cytb gene for cytochrome b. |
BaseCount: |
|
DNA-Sequence: |
X56295.txt |
AminoAcid-Sequence: |
X56295.txt |
PCR |
Name: |
WP2.2_ELISA-PCR_Myostatin |
Type: |
ELISA-PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Sequence: |
X56295 |
Genetic Element: |
Myostatin |
Ampl.Length: |
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GenomSize: |
|
AnnTemp: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
MY-r-long |
Antisense |
CCAAA TACCA gTgCC TgggT TCA |
23 |
72.1 |
63.4 |
70 |
52.2 |
2558-2580 |
BIO_MY-f |
Sense |
BIO_T TgTgC AAATC CTgAg ACCAT |
21 |
64.8 |
55.5 |
60 |
42.9 |
2480-2501 |
Organisation Data |
Acronym: |
TIB
|
|
Phone: |
+39-010-3628388 |
Partner Number: |
11 |
Fax: |
+39-010-3621928 |
Type: |
Company |
e-Mail: |
tib-molbiol@vega.cba.unige.it |
Name: |
Tib Molbiol |
Website: |
http://www.tib-molbiol.com/ |
Umbrella Organisation: |
|
Postal Address: |
c/o Tib Molbiol S.R.L., Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10; I-16132, Genova
c/o Centro Biotecnologie Avanzate
Largo Rosanna Benzi 10
16132 Genova, Italy
Largo Rosanna Benzi 10
16132 Genova, Italy |
Country: |
Italy |
Remarks: |
Task Leader Workpackage 2.2 and 2.3 |
Samples |
FoodName |
Extraction |
Process |
cNucl |
cDNA |
aDNA |
Supplier |
Muscle meat from 7 different animal species |
NucleoSpinTissue |
NoData |
5 - 69 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
InviSorb |
NoData |
22 - 61 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Dneasy |
NoData |
15 - 79 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
NucleoSpinFood |
NoData |
26 - 132 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
E.Z.N.A |
NoData |
4 - 26 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Lyse |
NoData |
6 - 7 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
CTAB |
NoData |
83 - 306 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Triton-X100 |
NoData |
8 - 23 |
|
2 |
BFEL |
Salami (S2 from Partner 2, no heat treatment) |
CTAB |
no heat treatment |
52±8 |
80±16 |
103 |
VUP |
Salami (S2 from Partner 2, no heat treatment) |
GeneSpin |
no heat treatment |
22±1 |
42±10 |
103 |
VUP |
Salami (S2 from Partner 2, no heat treatment) |
InviSorb |
no heat treatment |
106±17 |
222±69 |
104 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
CTAB |
heat-treated at 117 °C |
28±7 |
97±15 |
102 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
GeneSpin |
heat-treated at 117 °C |
6±2 |
8±7 |
100 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
InviSorb |
heat-treated at 117 °C |
40±13 |
270±34 |
102 |
VUP |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
yes |
ABI 7700, LightCycler |
yes |
MS, Biometra, PE, Eppendorf |
yes |
ELISA plate reader, MS-spectrometers (Electrospray, Ion Trap, Maldi-TOF) |
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