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You are here: Core Forms > Search Overview > Overview Overview  Search all Records  First  Previous Entry No. of 31  Next  Last  Taxonomy Popular Name: Genus: Species: Class: Order: Family: Animals Sequence NCBI-Acc: X56295 Sequence Description: S.scrofa mitochondrion cytb gene for cytochrome b. BaseCount: DNA-Sequence: X56295.txt AminoAcid-Sequence: X56295.txt PCR Name: WP2.2 ELISA-PCR CytochromeB Type: ELISA-PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295 Genetic Element: Mitochondrial Cytochrome b Ampl.Length: GenomSize: AnnTemp: Primers PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position cons_back_G_ELISA Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174 cons_back_M_ELISA Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174 BIO_SIM Sense BIO-g ACCTC CCAgC TCCAT CAAAC ATCTC ATCTT gAgAA A 37 72.1 72.4 95 45.9 58-95 Bos_misC Probe_Antisense FL-CC CAggA gggAA CCgCA A 18 71.7 62.9 60 66.7 14609-14626 Sus_misC_C Probe_Antisense FL-TA ggATT TgCAA gATCA ggCCg ATg 25 72.1 64.3 74 48 57-81 Ovi_A_ELISA Probe_Antisense FL-TA gAATC TgTAA AATTA AgCAA ATgCC TAg 30 66.8 58.1 78 30 109-138 Equ_misA_G_A Probe_Antisense FL-gT TAAgA TTTgg AggAT AAggg AgAAT 27 66.3 58.5 74 37 57-83 Publications Short Title Type Date Download Rep: WP2.2_ELISA_PCR_TibMolbiol Report 2004 Rep_WP2.2_ELISA_PCR_TibMolbiol.pdf Organisation Data Acronym: TIB Phone: +39-010-3628388 Partner Number: 11 Fax: +39-010-3621928 Type: Company e-Mail: tib-molbiol@vega.cba.unige.it Name: Tib Molbiol Website: http://www.tib-molbiol.com/ Umbrella Organisation: Postal Address: c/o Tib Molbiol S.R.L., Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10; I-16132, Genova c/o Centro Biotecnologie Avanzate Largo Rosanna Benzi 10 16132 Genova, Italy Largo Rosanna Benzi 10 16132 Genova, Italy Country: Italy Remarks: Task Leader Workpackage 2.2 and 2.3 Persons Title / Name e-Mail Phone Remarks Dr. Davide Bini dbini@tibmolbiol.com; tib-molbiol@vega.cba.unige.it +39-010-3628388 Dr. Elisabetta Carpaneto ecarpaneto@tibmolbiol.com +39-010-3628388 Dr. Olfert Landt OLandt@tib-molbiol.de +49-30-787994-44 Syntheselabor Dr. Eduardo Thueroff ethueroff@tibmolbiol.com +39-010-3628388 Samples FoodName Extraction Process cNucl cDNA aDNA Supplier Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP Devices Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other yes ABI 7700, LightCycler yes MS, Biometra, PE, Eppendorf yes ELISA plate reader, MS-spectrometers (Electrospray, Ion Trap, Maldi-TOF) You are here: Core Forms > Search Overview > Overview © Copyright MolSpec Disclaimer: MolSpec is not responsible for linked contents.