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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Animals |
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PCR |
Name: |
DloopSeq |
Type: |
Real-Time PCR |
System: |
Sequencing |
Sequence: |
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Genetic Element: |
D-Loop |
Ampl.Length: |
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GenomSize: |
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AnnTemp: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
DloopSeq F |
Sense |
CCTCT ggTTC CTCgg TCAgg |
20 |
69.5 |
60.8 |
66 |
65.0 |
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DloopSeq R |
Antisense |
CCAAA gTgCA TCAgT gTCAA ggTgA T |
26 |
71.9 |
63.1 |
76 |
46.2 |
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Organisation Data |
Acronym: |
TUGraz
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|
Phone: |
+43-316 873 6471 |
Partner Number: |
12 |
Fax: |
+43-316 8736971 |
Type: |
University |
e-Mail: |
ursula.muelleder@tugraz.at |
Name: |
Institute of Bio- and Food Chemistry, TUGraz |
Website: |
http://www.cis.tugraz.at/ilct/ |
Umbrella Organisation: |
University of Technology Graz |
Postal Address: |
TUGraz -
Institute of Bio- and Food Chemistry
Department of Food Chemistry and Technology
Petersgasse 12/II, A-8010, Graz
8010 Graz, Austria |
Country: |
Austria |
Remarks: |
Workpackages 1.3, 1.4, 2.1, 3 and 4 |
Samples |
FoodName |
Extraction |
Process |
cNucl |
cDNA |
aDNA |
Supplier |
Muscle meat from 7 different animal species |
NucleoSpinTissue |
NoData |
5 - 69 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
InviSorb |
NoData |
22 - 61 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Dneasy |
NoData |
15 - 79 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
NucleoSpinFood |
NoData |
26 - 132 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
E.Z.N.A |
NoData |
4 - 26 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Lyse |
NoData |
6 - 7 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
CTAB |
NoData |
83 - 306 |
|
3 |
BFEL |
Muscle meat from 7 different animal species |
Triton-X100 |
NoData |
8 - 23 |
|
2 |
BFEL |
Salami (S2 from Partner 2, no heat treatment) |
CTAB |
no heat treatment |
52±8 |
80±16 |
103 |
VUP |
Salami (S2 from Partner 2, no heat treatment) |
GeneSpin |
no heat treatment |
22±1 |
42±10 |
103 |
VUP |
Salami (S2 from Partner 2, no heat treatment) |
InviSorb |
no heat treatment |
106±17 |
222±69 |
104 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
CTAB |
heat-treated at 117 °C |
28±7 |
97±15 |
102 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
GeneSpin |
heat-treated at 117 °C |
6±2 |
8±7 |
100 |
VUP |
Pâté (S3 from Partner 2, heat-treated at 117 °C) |
InviSorb |
heat-treated at 117 °C |
40±13 |
270±34 |
102 |
VUP |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
yes |
ABI 7700 |
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ABI 9600, ABI 9700 |
yes |
ELISA plate reader Anthos, UV-VIS Varian |
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