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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Cattle |
Bos |
taurus |
Eutheria |
Ruminantia |
Bovidae |
Sequence |
NCBI-Acc: |
M27541 |
Sequence Description: |
Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds. |
BaseCount: |
887 a 783 c 798 g 733 t |
DNA-Sequence: |
M27541.txt |
AminoAcid-Sequence: |
M27541.txt |
PCR |
Name: |
Bos-pde |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
M27541 |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
154 |
GenomSize: |
|
AnnTemp: |
60 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-pde-f |
Sense |
ACACA ATgAA TTgAC TCCTA CCC |
23 |
64.1 |
55.4 |
66 |
43.5 |
2994-3016 |
Bos-pde-r |
Antisense |
ggTgC gTAAT ggAgg AgTC |
19 |
63.1 |
54.3 |
60 |
57.9 |
3147-3129 |
Bos-pde-probe |
Marker/Probe |
ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC |
33 |
71.9 |
63.1 |
86 |
30.3 |
3124-3092 |
Bos-pde-Amplicon |
Amplicon |
acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC |
154 |
60 |
87.5 |
420 |
36.4 |
2994-3147 |
Organisation Data |
Acronym: |
BfR
|
|
Phone: |
+49-30-8412-0 |
Partner Number: |
1 |
Fax: |
+41 308412 3685 |
Type: |
Authority |
e-Mail: |
j.zagon@bfr.bund.de |
Name: |
BfR-Federal Institute for Risk Assessment, Berlin |
Website: |
http://www.bfr.bund.de/ |
Umbrella Organisation: |
Bund, Germany (Bundesinstitut für Risikobewertung) |
Postal Address: |
Thielallee 88-92, D-14195 Berlin |
Country: |
Germany |
Remarks: |
Coordinator MolSpec-ID-project, Task Leader Workpackages 1.4 and 4 |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
DNA+protein |
ABI 7700+LC |
ABI 310 |
PE 2400 |
yes |
ELISA plate reader |
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