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Taxonomy |
Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
Wheat |
Triticum |
aestivum L. |
Liliopsida |
Poales |
Poaceae alt. Gramineae |
Barley |
Hordeum |
vulgare |
Liliopsida |
Poales |
Pocaceae |
Rye |
Secale |
cereale |
Liliopsida |
Poales |
Pocaceae |
PCR |
Name: |
WBR |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Sequence: |
|
Genetic Element: |
Intron of trnL gene |
Ampl.Length: |
|
GenomSize: |
|
AnnTemp: |
|
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
WBR11 |
Sense |
ggTAA CTTCC AAATT CAgAg AAAC |
24 |
62.7 |
53.9 |
66 |
37.5 |
|
WBR13 |
Antisense |
TCTCT AATTT AgAAT TAgAA ggAA |
24 |
55.7 |
46.9 |
60 |
25.0 |
|
Organisation Data |
Acronym: |
VUP
|
|
Phone: |
+421 2 55574622 |
Partner Number: |
5 |
Fax: |
+421 2 55571417 |
Type: |
Authority |
e-Mail: |
vup@vup.sk |
Name: |
VUP-Food Research Institute |
Website: |
http://www.vup.sk/ |
Umbrella Organisation: |
|
Postal Address: |
Vyskumny Ustav Potravinarsky
Priemyselna 4
PO Box 25
SK-82475 Bratislava 26, Slovakia |
Country: |
Slovakia |
Remarks: |
Task Leader Workpackage 1.2 |
Samples |
FoodName |
Extraction |
Process |
cNucl |
cDNA |
aDNA |
Supplier |
Wheat flour |
CTAB |
flour |
177±36 |
374±20 |
103 |
VUP |
Wheat flour |
GeneSpin |
flour |
706±83 |
918±152 |
104 |
VUP |
Wheat flour |
InviSorb |
flour |
85±26 |
479±140 |
102 |
VUP |
Wheat biscuit |
CTAB |
biscuit |
65±26 |
85±23 |
103 |
VUP |
Wheat biscuit |
GeneSpin |
biscuit |
26±3 |
55±2 |
102 |
VUP |
Wheat biscuit |
InviSorb |
biscuit |
156±67 |
146±40 |
103 |
VUP |
Wheat biscuit with chocolate (Twix) |
CTAB |
biscuit with chocolate (and caramel; Twix) |
34±13 |
31±1 |
102 |
VUP |
Wheat biscuit with chocolate (Twix) |
GeneSpin |
biscuit with chocolate (and caramel; Twix) |
26±6 |
23±8 |
102 |
VUP |
Wheat biscuit with chocolate (Twix) |
InviSorb |
biscuit with chocolate (and caramel; Twix) |
56±7 |
30±12 |
102 |
VUP |
Wheat Brei (Instant) |
CTAB |
Instant Brei |
30±14 |
86±10 |
102 |
VUP |
Wheat Brei (Instant) |
GeneSpin |
Instant Brei |
30±3 |
55±1 |
102 |
VUP |
Wheat Brei (Instant) |
InviSorb |
Instant Brei |
56±18 |
37±10 |
102 |
VUP |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
yes |
ABI 7000 in 2002 |
(only subcontractor) |
ABI 9700 |
(only subcontractor) |
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