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PCR |
Name: |
Chicken |
Type: |
Real-Time PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
D-Loop |
Ampl.Length: |
|
GenomSize: |
1.08 |
AnnTemp: |
|
Taxonomy: |
Chicken |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
|
nPrimers: |
3 |
Organisation: |
TUGraz |
Validation: |
NoValidationAvailable |
Sequence: |
AY235571 |
Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Chicken F |
Sense |
AATCA CgACC ACCTT ACAAC CTTAC |
25 |
67.3 |
58.6 |
72 |
44.0 |
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Chicken R |
Antisense |
AAAAT gTCgA CCAgg ggTTT ATg |
23 |
68.3 |
59.6 |
66 |
43.5 |
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Chicken MGB |
Marker/Probe |
AgCCC CACTg ggTC |
14 |
59.3 |
50.6 |
48 |
71.4 |
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Sequence |
NCBI-Acc: |
AY235571 |
Sequence Description: |
Gallus gallus mitochondrion, complete genome. |
BaseCount: |
5075 a 5456 c 2270 g 3981 t |
DNA-Sequence: |
AY235571.txt |
AminoAcid-Sequence: |
AY235571.txt |
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